袁 丽 鲁 涤 石美森 杨 雪

【摘 要】 目的用复合荧光扩增体系调查辽宁鞍山岫岩满族无关个体 D6S1043、D7S3048、D9S925、D11S2368、D14S608、D15S659、D17S1290、D20S470 和 GATA198B05 等9个STR基因座的遗传多态性。方法用本实验室构建的 9 个常染色体STR基因座荧光复合扩增体系，对辽宁鞍山岫岩满族 252 个无关个体的DNA进行PCR扩增，3130型基因分析仪电泳检测扩增产物及等位基因分型标准物，GeneMapper誖3.2分析软件中导入本体系Panel和Bin，对电泳结果进行分析，按照重复序列重复次数命名等位基因，使用 PowerStatsV12 和 GENEPOP 软件进行统计学分析。结果9个STR基因座在辽宁鞍山岫岩满族基因型频率分布均符合 Hardy-Weinberg 平衡（P﹥0.05）,多态性信息含量在 0.750～0.860 之间，杂合度在 0.794～0.881 之间，个体识别力在 0.918～0.968 之间，非父排除概率在 0.587～0.757 之间，累积非父排除率为 0.999 96，累积个体识别能力为 0.999 999 999 999 1。结论 这 9 个非CODIS系统STR基因座在鞍山岫岩满族群体中等位基因频率分布均匀，多态性好，对群体遗传学研究和进行法医学应用具有重要意义，可以作为现有商品化试剂的有益 STR 补充。

【关键词】 法医生物学；短串联重复序列；遗传多态性；岫岩满族

【中图分类号】 D919.2

【文献标识码】 A

【文章编号】 1674-1226（2011）05-0632-05

Genetic Polymorphism of 9 CODIS Short Tandem Repeat Loci in Xiuyan Manchu Population. Yuan Li, Lu Di, Shi Meisen, et al. Shanghai Key Lab of Forensic Medicine，Shanghai 200063

【Abstract】 Objective To investigate the genetic polymorphisms of 10 short tandem repeats loci (D6S1043,D7S3048, D9S925, D11S2368, D14S608, D15S659, D17S1290, D20S470 and GATA198B05) in Liaoning Anshan Xiuyan Man population. Methods We used a multiplex system developed by ourselves to amplify 252 Xiuyan Man population unrelated individual’s DNA. The amplified fragments were separated by electrophoresis in 3130 Genetic Analyzer. We used GeneMapper R3.2 software to analyze these results. Results The distributions of genotype for 9 STR loci in the population were all in accordance with Hardy-Weinberg equilibrium. The polymorphism information component of the 9 STR loci was among 0.750 to 0.860; the heterozygote was among 0.794 to 0.881;the discrimination power was among 0.918 to 0.968; the probability of exclusion wase among 0.587 to 0.757; the combined probability of exclusion was 0.999 96; and the combined discrimination power was 0.999 999 999 9991. Conclusion The 9 STR loci have high probability of exclusion and discrimination power，which can be loci available as the candidate genetic markers for population genetics and forensic practice.

【Key Words】 Forensic biology， Short tandem repeats， Genetic polymorphism，Xiuyan Manchu Population